PLASMID FOR EXPRESSING SECRETION OF POLYPEPTIDE USABLE IN MOLD AND YEAST AND PRODUCTION OF POLYPEPTIDE USING THE SAME

PLASMID FOR EXPRESSING SECRETION OF POLYPEPTIDE USABLE IN MOLD AND YEAST AND PRODUCTION OF POLYPEPTIDE USING THE SAME

PURPOSE:To provide a new plasmid capable of secreting and expressing a useful protein and peptide such as a physiologically active peptide, a hormone or an enzyme by a mold or a yeast. CONSTITUTION:This plasmid has a promoter capable of functioning with a mold and a yeast, further a signal and a ter...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: YAMAGUCHI SHOTARO, WASHIZU KINYA
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE:To provide a new plasmid capable of secreting and expressing a useful protein and peptide such as a physiologically active peptide, a hormone or an enzyme by a mold or a yeast. CONSTITUTION:This plasmid has a promoter capable of functioning with a mold and a yeast, further a signal and a terminator required for the secretion and a DNA region, replicable and selectable in Escherichia coli and is operable with the mold and yeast, e.g. a plasmid pY4'. The exemplified plasmid is constructed according to a scheme shown in the figure and a plasmid pLGR80 is obtained by ligation of a plasmid pLGG300 to a 0.63-kb Xhol/Ncol fragment derived from a cDNA. A plasmid pLGG100, the plasmid pLGG300 and an about 1.0-kb cDNA fragment are prepared by inserting a mono-and a diacylglycerol lipase gene 2.0-kb HindIII fragment of Penicillium camembertii U-150 strain into a HindIII site of an Escherichia coli vector pUC19.