MANUFACTURE OF BOND MATRIX, METHOD FOR MEASURING SPECIMEN IN SAMPLE SOLUTION, LAYER THAT CAN BE REGENERATED AND NEW COMPOUND

PURPOSE: To obtain a binding matrix forming a binding layer dilute and laterally uniform in the content of a solid phase reactant by incubating a carrier substance along with the solid phase reactant bonded to a fixing group through a short chain spacer molecule and a water-soluble reaction soln. containing a hydrophilic diluting molecule. CONSTITUTION: As an aq. reaction soln., water containing no org. solvent or an aq. buffering soln. system is used. The carrier layer 22 of a binding matrix is formed by applying a thin chromium layer 23a and a gold layer 23b to the surface of an optically transparent substance by vapor deposition and a solid phase reactant 26 is adsorbed on the gold layer 23b by a fixing group such as thiol through a short chain spacer 25. A free reactant 27 is bondable to the solid phase reactant 26 and present in a solid phase 28. By incubating the solid phase reactant 26 and the aq. reaction soln., a dilute bonding matrix uniform in its lateral direction is formed and can be detected optically. That is, the optical thickness of each layer is measured from the reflected light of a test part 20 by a Kretschmann's apparatus and the amt. of an antibody in a specimen can be detected.