METHOD FOR DISCRIMINATION OF SEX IN MURINE AND BOVINE EMBRYO UTILIIZNG GENE HAVING SEXUAL DIFFERENCE IN EXPRESSION

PURPOSE:To obtain a method for discriminating the sex of a murine or a bovine embryo or tissue utilizing a gene having a difference in expression between the female and the male and a primer or a probe therefor. CONSTITUTION:The method for discriminating the sex utilizes a cDNA of a murine and a bov...

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Hauptverfasser:	KONDO MASAAKI, ITAGAKI YOSHIAKI, SATO SEIJI, SUDOU SHIZUYO, NAKAMURA TOYORO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DIAGNOSIS ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE IDENTIFICATION INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SURGERY TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To obtain a method for discriminating the sex of a murine or a bovine embryo or tissue utilizing a gene having a difference in expression between the female and the male and a primer or a probe therefor. CONSTITUTION:The method for discriminating the sex utilizes a cDNA of a murine and a bovine Mea genes as a gene having a difference in expression between the female and the male, a genome DNA of the bovine Mea gene, an RNA and a polypeptide designated therewith and a part of the nucleic acids as a primer according to the RT-PCR method (polymerase chain reaction utilizing a reverse transcriptase). The probe for discriminating the sex by the hybridization method utilizing all or a part of the nucleic acids is obtained. Furthermore, the polypeptide to be an antigen for discriminating the sex by the antigen- antibody reaction is provided.