HEAT SHOCK PROTEIN GENE AND PRODUCTION OF HEAT SHOCK PROTEIN

PURPOSE:To prevent the denaturation of protein, regenerate the denaturated protein and improve the productivity of protein by utilizing danK gene coding a heat shock protein. CONSTITUTION:A danK gene coding a heat shock protein DanK originated from a thermophilic Bacillus stearothermophilus such as...

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Hauptverfasser:	TSUJI YUJI, OUDA TOYOO, IMANAKA TADAYUKI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To prevent the denaturation of protein, regenerate the denaturated protein and improve the productivity of protein by utilizing danK gene coding a heat shock protein. CONSTITUTION:A danK gene coding a heat shock protein DanK originated from a thermophilic Bacillus stearothermophilus such as Bacillus stearothermophilus SIC1 strain is cloned. The danK gene is constructed of 1,809bp and codes 603 amino acids. The cloning of the danK gene can be carried out by introducing a DNA fragment originated from thermophilic microorganism into a vector, transforming a host microorganism with the prepared plasmid and culturing the transformant.