SORBITOL OXIDASE, ITS PRODUCTION AND USE THEREOF

PURPOSE:To obtain a new sorbitol oxidase, capable of acting on D-sorbitol, consuming oxygen and producing hydrogen peroxide, good in stability and useful as a reagent, etc., for measuring the sorbitol content in erythrocytes to be an index of diabetic complications. CONSTITUTION:A microorganism, hav...

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Hauptverfasser:	AISUI SHIGENORI, HATTORI SHIZUO, SOGABE YUKIHIRO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To obtain a new sorbitol oxidase, capable of acting on D-sorbitol, consuming oxygen and producing hydrogen peroxide, good in stability and useful as a reagent, etc., for measuring the sorbitol content in erythrocytes to be an index of diabetic complications. CONSTITUTION:A microorganism, having the ability to produce a sorbitol oxidase and belonging to the genus Xanthomonas [e.g. Xanthomonas maltophilia TE3539 (FERM P-13296)] is inoculated into a culture medium and cultured at 30 deg.C and the resultant culture solution is used as a seed culture solution and subsequently transferred to a jar fermenter and cultured at 30 deg.C. The obtained culture solution is centrifuged to collect microbial cells, which are then subjected to pressing treatment and centrifugation. A supernatant is subsequently collected and purified by the fractionation with ammonium sulfate, anion exchange chromatography and gel filtration, etc., to afford the objective new sorbitol oxidase capable of acting on D-sorbitol, consuming oxygen and producing hydrogen peroxide. Furthermore, the resultant new sorbitol oxidase is made to act on a sample containing the sorbitol, etc., to measure the amount of the produced hydrogen peroxide. Thereby, the amount of the sorbitol is measured.