PRODUCTION OF PHOSPHOLIPASE A

PRODUCTION OF PHOSPHOLIPASE A

PURPOSE:To industrially and advantageously obtain phospholipase A by (culturing an alga of the genus Euglena having the ability to produce the phospholipase A in a culture medium containing a C and an N sources. CONSTITUTION:An alga of the genus Euglena (preferably Euglena gracilis) having the abili...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: IWASAKI TAISUKE, FUJITA TAKASHI
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE:To industrially and advantageously obtain phospholipase A by (culturing an alga of the genus Euglena having the ability to produce the phospholipase A in a culture medium containing a C and an N sources. CONSTITUTION:An alga of the genus Euglena (preferably Euglena gracilis) having the ability to produce phospholipase A is cultured in a culture medium containing a C source (preferably glucose), an N source (preferably glutamic acid or diammonium hydrogenphosphate) at 4-35 ratio (C/N) under conditions of preferably pH 3.0-4.5, 20-32 deg.C culture temperature and irradiation with light or in the dark for 3-7 days to produce and accumulate the phospholipase A in the organism. The resultant phospholipase A is then separated and collected to afford the objective phospholipase A.