JPH05339295

PURPOSE:To produce an antibody by bonding the objective peptide to the carboxyl terminal of dihydrofolic acid reductase(DHFR) and using the produced fused protein as an immunizing antibody. CONSTITUTION:The objective process for the preparation of an antibody against a peptide is composed of a proce...

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Bibliographische Detailangaben
Hauptverfasser: OHASHI SHINICHI, IWAKURA MASAHIRO, KOKUBU TOMOKUNI
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:PURPOSE:To produce an antibody by bonding the objective peptide to the carboxyl terminal of dihydrofolic acid reductase(DHFR) and using the produced fused protein as an immunizing antibody. CONSTITUTION:The objective process for the preparation of an antibody against a peptide is composed of a process for producing a DHFR fused protein having the objective peptide on the carboxyl terminal side by genetic engineering technique, a process for separating the objective fused protein from the host microorganism and purifying the protein and a process for immunizing an animal using the objective highly purified fused protein.