PRELIMINARY TREATMENT OF B12 ENZYME IMMUNOASSAY AND SAMPLE

PURPOSE: To obtain the provision of an enzyme immunoassay having a focus at the assay of vitamin B12 . CONSTITUTION: The denaturant used to pretreat a test sample for immunoassay is neutralized and inactivated by adding treating agent of the combination of acid buffer and reagent for converting the...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	MAAKU EE SUTAPURESU, JIYON EMU POSUKII, MAIKERU AI WATOKINSU, EDOWAADO TOUNNUU RIAN, KURIFUOODO AARU BAATORETSUTO
Format:	Patent
Sprache:	eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PURPOSE: To obtain the provision of an enzyme immunoassay having a focus at the assay of vitamin B12 . CONSTITUTION: The denaturant used to pretreat a test sample for immunoassay is neutralized and inactivated by adding treating agent of the combination of acid buffer and reagent for converting the sulfhydryl group of the denaturant into inactivation type such as, for example, disulfide or alkylthio group. The treating agent is operated without denatured activity to disturb the sequential steps of this assay step. Further, the method for the assay of vitamin B12 using the pretreating agent is disclosed.