JPH0521551B

NEW MATERIAL:A double stranded plasminogen activator collected from the tissue culture liquid of the cell originated from the normal tissue of man. Molecular weight, 63,000+ or -10,000; isoelectric point, 7-8.5; optimum pH, 7-9.5; having affinity to fibrin and concanavalin A; decomposition by reduction; stability, stable at 55 deg.C for 3hr. USE:Thrombolytic agent, antithrombic agent for artificial vessel, etc., and diagnotic for thrombosis, etc. It has 30 times stronger thrombolytic activity than urokinase. It can be administered in the blood vessel, especially at the site of thrombus. Dose: 50-1,000,000 unit/day by intravenous injection. PREPARATION:The fraction having plasminogen activator (abbreviated as AP) activity is separated from the tissure culture liquid of the normal human tissue (e.g. kidney, intestine, lung, etc. of human fetus). The fraction is subjected to the enzymatic treatment or incubated at 10-80 deg.C, preferably 20-40 deg.C, and the product is separated and purified to obtain the objective double stranded AP.