NEW PLASMID, MICROBE, ANTIALLERGIC CHIMERAL PROTEIN AND ITS PRODUCTION

PURPOSE:To provide a new plasmid useful for producing antiallergic chimeral protein. CONSTITUTION:The objective plasmid comprising (I) the first DNA domain composed of (A) a DNA sequence coding antiallergic chimeral protein having an amino acid sequence of the formula, (B) a second DNA sequence havi...

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Bibliographische Detailangaben
1. Verfasser:	KITAI KAZUO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To provide a new plasmid useful for producing antiallergic chimeral protein. CONSTITUTION:The objective plasmid comprising (I) the first DNA domain composed of (A) a DNA sequence coding antiallergic chimeral protein having an amino acid sequence of the formula, (B) a second DNA sequence having promoter function and (C) a third DNA sequence coding signal peptide and (II) the second DNA domain composed of (D) a fourth DNA sequence imparting host cells with extracellular secretion-promoting activity and (E) a fifth DNA sequence having promoter function; e.g. plasmid pEG2. This plasmid pEG2 can be obtained by introducing (1) a DNA fragment obtained by linking a chemically synthesized human IgEFc domain gene to cloned IgGFc domain gene into (2) a plasmid having (a) appropriate promoter domain-signal peptide domain- IgGFc domain, (b) Ex promoter domain derived from antialkaline bacillus No.170 strain and (c) Kil gene derived from pMB9 plasmid.