METHOD OF INFECTION OF FISH WITH EDWARDSIELLA TARDA

PURPOSE:To accomplish the title infection with desired infection and death rates by raising fish in raising water containing Edwardsiella tarda culture filtrate, ammonia and Edwardsiella tarda. CONSTITUTION:Fish is raised in raising water containing (A) 0.5 - 1l/100l of a residual solution obtained by separating solids such as Edwardsiella tarda from a Edwardsiella tarda culture solution, (B) 0.5 - 10 ppm of ammonia, (C) 0.1 - 1.0 ppm of at least one kind of nitrite (pref. sodium, potassium or ammonium sat) and (D) 10 - 10 /ml of Edwardsiella tarda. With the present method, artificial infection can be made in a simple operation without causing fish injuries and putting the fish to unnecessary stressed state; and by regulating the raising conditions, the system can be easily controlled to infectious state adapted to the purpose of experiments, thus being suitable as an experimental system for developing medicines effective for the prevention and therapy of the diseases induced by Edwardsiella tarda.