METHOD FOR EXAMINING SENSITIVITY OF MICROORGANISM TO DRUG

PURPOSE:To carry out test of sensitivity of a microorganism to drug in a remarkably short time and high precision by culturing a microorganism to be examined in a drug-containing medium and then measuring an amount of adenosine triphosphate in microbial cell of the microorganism. CONSTITUTION:A micr...

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Bibliographische Detailangaben
Hauptverfasser:	KANETO REI, KII YOSHIAKI, OKAMOTO ROKURO, SAKAMOTO MICHIKO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To carry out test of sensitivity of a microorganism to drug in a remarkably short time and high precision by culturing a microorganism to be examined in a drug-containing medium and then measuring an amount of adenosine triphosphate in microbial cell of the microorganism. CONSTITUTION:A microorganism to be examined is aseptically inoculated into a medium containing a drug such as antibiotic and cultured. Then an amount of adenosine triphosphate(ATP) in microbial cell of the cultured microorganism is measured and the measured ATP amount is compared with reference ATP amount in culture start. Measurement of ATP amount of microbial cell to be examined is preferably carried out by measuring of luminescent amount of luciferin-luciferase system used. A system derived from a firefly is preferably used as the luciferin-luciferase system.