ANALYSIS METHOD USING TETRASORIUM SALT INDICATOR HAVING REFLECTANCE PLATEAU
PURPOSE: To improve accuracy in the analytical assay for measuring a chromogenic dyeing material by employing a specified thizolyltetrazolium salt as an indicating reagent. CONSTITUTION: As an indication reagent, produced formazan transmits a reflection spectrum indicating a wide plateau exceeding a...
Gespeichert in:
Hauptverfasser: | , , , , |
---|---|
Format: | Patent |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | PURPOSE: To improve accuracy in the analytical assay for measuring a chromogenic dyeing material by employing a specified thizolyltetrazolium salt as an indicating reagent. CONSTITUTION: As an indication reagent, produced formazan transmits a reflection spectrum indicating a wide plateau exceeding about 600nm. Thizolyltetrazolium salt shown by formula A is employed. Since a reflection spectrum indicating a wide plateau exceeding 600nm is provided, a test sample is caused to react on an analyte and a test composition containing a reducing material, e.g. a reagent producing NADH, and an indicating reagent compound being reduced by the reducing material to produce a chromogenic dyeing material is brought into contact with a test tool containing a mixed solid carrier matrix. The carrier matrix is irradiated from a light source and the reflected light is measured by a detector arrangement thus detecting the reflectance of the carrier matrix. According to the arrangement, accuracy can be improved in the analytical assay for measuring a chromogenic dyeing material. |
---|