SIMPLE ENZYME IMMUNOASSAY OF TISSUE PLASMINOGEN ACTIVATOR

PURPOSE:To measure simple enzyme immunity of tissue plasmonogen activator (t-PA) without subjecting a plasma sample to an acid treatment by using a polyclonal PC antibody for an insolubilized antibody and antibody labeled with an enzyme. CONSTITUTION:The t-PA which is an antigen is conjugated with the PC antibody insolubilized on a carrier and the PC antibody labeled with the enzyme is also conjugated with the antigen to form a complex. The labeling quantity thereof is measured. The PC antibody is prepd. by dissolving, for example, the t-Pa in a physilogical salt soln., then mixing the soln. with Freund's complete adjuvant, immunizing an animl several times with this soln. to obtain anti-t-Pa anti-serum and salting out this serum to obtain the anti-t-Pa antibody globulin. An active carbon is properly used in addition to magnetic material particles, glass, etc., as the carrier to immobilize the antibody. All the enzymes normally used for EIA are included in the enzyme. The antibody labeled with the enzyme is prepd. by using various protein crosslinking agents. The quantity of the t-Pa which is the antigen is exactly measured in accordance with the common method by measuring this label.