METHOD FOR PREPARATION OF L-PHOSPHINOTHRICIN BY CONJUGATE ENZYMATIC REACTION

A coupled enzymatic reaction of a transaminase with GOT activity and of a transaminase with L-phosphinothricin transaminase activity produces phosphinothricin in virtually quantitative yield and virtually without any contamination by a natural amino acid when the amino donor glutamate is employed in...

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Bibliographische Detailangaben
Hauptverfasser:	GERUTO FUYURINGU, KURAUSU BAARUCHIYU, ARUNO SHIYURUTSU
Format:	Patent
Sprache:	eng
Schlagworte:	ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A coupled enzymatic reaction of a transaminase with GOT activity and of a transaminase with L-phosphinothricin transaminase activity produces phosphinothricin in virtually quantitative yield and virtually without any contamination by a natural amino acid when the amino donor glutamate is employed in catalytic amounts and the amino donor aspartate is employed in approximately equimolar amounts based on HMPB.