QUANTITATIVE ANALYSIS OF POLYAMINE

PURPOSE:To quantitatively analyze a polyamine readily and high sensitively by acting a polyamine oxidase on a sample, then acting catalase thereon in the presence of an alcohol, subsequently acting a dehydrogenase in the presence of NAD and measuring the generated NADH. CONSTITUTION:A polyamine oxid...

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Bibliographische Detailangaben
Hauptverfasser:	AISUI SHIGENORI, SOGABE YUKIHIRO, MINAMI MICHIYO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To quantitatively analyze a polyamine readily and high sensitively by acting a polyamine oxidase on a sample, then acting catalase thereon in the presence of an alcohol, subsequently acting a dehydrogenase in the presence of NAD and measuring the generated NADH. CONSTITUTION:A polyamine oxidase (e.g. putrescine oxidase) is acted on a polyamine (e.g. putrescine)-containing sample to convert the polyamine to hydrogen peroxide and an aminoalkylaldehyde. Catalase is acted on the generated hydrogen peroxide in the presence of an alcohol (e.g. ethanol) to convert the alcohol to aldehydes and aminoalkylaldehyde dehydrogenase is subsequently acted on the generated aldehyde and aminoalkylaldehyde in the presence of NAD and NADP. The generated NADH or NADPH is analyzed, e.g. by measuring the absorption at 340nm using a spectrometer, thus quantitatively analyzing the objective polyamine.