AGENT FOR TREATMENT AND PREVENTION OF HYPERCALCEMIA

PURPOSE:To obtain the subject new treating and preventing agent capable of curing hypercalcemia in highly desirable result over a relatively long period by using an anti-human parathyroid hormone-relating protein monoclonal antibody or/its relating substance as active component. CONSTITUTION:A hybri...

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Hauptverfasser:	NAGAOKA TETSUYA, ASAHI KOJI, EGUCHI YUKIO, KUNIHIRO SHIGEKI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To obtain the subject new treating and preventing agent capable of curing hypercalcemia in highly desirable result over a relatively long period by using an anti-human parathyroid hormone-relating protein monoclonal antibody or/its relating substance as active component. CONSTITUTION:A hybridoma is prepared by mixing a human parathyroid hormone-relating protein (PTHrP) with Freund's complete adjuvant, immunizing a BALB mouse with the mixture by subcutaneous injection, collecting immunized splenocytes after booster immunization, fusing the cell with a mouse myeloma cell in the presence of polyethylene glycol and culturing the fused cell on HAT medium. The hybridoma is screened to select a clone capable of producing anti-PTHrP antibody, which is cloned by limiting dilution analysis, etc., to obtain a monoclonized hybridoma. The hybridoma is cultured to obtain an anti-PTHrP monoclonal antibody. The objective agent for the treatment or prevention of hypercalcemia can be prepared by using the antibody or its relating substance such as F(ab') fragment as active component.