IMMUNE MEASUREMENT

PURPOSE:To enable detection of an antibody with high reproducibility by incorporating a process wherein a ferritin-labeled antibody forms color in reaction with hinokitiol to eliminate variations in results as caused by a measuring person or the like with a handy operation. CONSTITUTION:A ferritin-l...

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Bibliographische Detailangaben
Hauptverfasser: TAMAGAWA KANNA, MIYAKOSHI HIDEO
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:PURPOSE:To enable detection of an antibody with high reproducibility by incorporating a process wherein a ferritin-labeled antibody forms color in reaction with hinokitiol to eliminate variations in results as caused by a measuring person or the like with a handy operation. CONSTITUTION:A ferritin-labelled antibody can be made by a method used in a ferritin antibody method and that available commercially is usable. A reaction between the antibody and a hinokitiol aqueous solution is accomplished for about 1-120 minute at a room temperature. A sample can be used without dilution and an antigen to be measured is not limited. For example, a ferritin- labelled anti-human IgG (concentration of protein of 6mg/ml) diluted by 2 times is put into a well of a microtiter plate and 0.025 ml of a 0.5% hinokitiol aqueous solution is put into each well to cause a reaction for one minute at the room temperature. This enables observation of a color of red formed at a concentration exceeding 15 mug/ml (400 fold dilution) in the concentration of the antibody.