AMPLIFYING METHOD OF NUCLEIC ACID SEQUENCE AND CLONING METHOD OF SAME SEQUENCE

PURPOSE:To safely detect specific nucleic acid in high sensitivity by making nucleic acid in a sample to linear double-chains DNA, adding synthetic linker and amplifying gene with DNA-polymerase using primer complementary to base sequence of the linker. CONSTITUTION:In amplifying a very small amount...

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Bibliographische Detailangaben
Hauptverfasser:	ARAKI MASAYASU, ICHIKAWA MUNETAKA, HAMADA FUKUSABURO, MIYAZAKI JUNICHI, YAMAMURA KENICHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To safely detect specific nucleic acid in high sensitivity by making nucleic acid in a sample to linear double-chains DNA, adding synthetic linker and amplifying gene with DNA-polymerase using primer complementary to base sequence of the linker. CONSTITUTION:In amplifying a very small amount of nucleic acid sequence contained in a sample, nucleic acid in the sample is made to a shape of linear double-chains DNA and synthetic linker constructing double-chains DNA having one even end and the other protruding end derived from annealing of single chain synthetic DNA is added to said nucleic acid sequence, then gene-amplifying reaction is performed by PCR(Polymerase chain reaction) using DNA polymerase with using primer having base sequence complementary to the base sequence of said linker part to amplify nucleic acid sequence, in vitro.