METHOD FOR COUNTING LIVING MICROBIAL CELLS

PURPOSE:To count the number of living microbial cells in high sensitivity by adding a fluorescein derivative dissolved in a solvent to a specimen, keeping the mixture at a prescribed temperature for a prescribed time, lowering the pH of the mixture with an acid and irradiating the specimen with an e...

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Bibliographische Detailangaben
Hauptverfasser:	UEDA RYOHEI, SUGATA KIYOSHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To count the number of living microbial cells in high sensitivity by adding a fluorescein derivative dissolved in a solvent to a specimen, keeping the mixture at a prescribed temperature for a prescribed time, lowering the pH of the mixture with an acid and irradiating the specimen with an excitation light. CONSTITUTION:A fluorescein derivative dissolved in a solvent is added to a specimen and maintained at a prescribed temperature for a prescribed time. The pH of the mixture is lowered with an acid, the specimen is irradiated with an excitation light and the number of the living microbial cells is counted by detecting the fluorescence emitted from the cell. As an alternative method, a fluorescein derivative dissolved in a solvent and free from dilution with a phosphoric acid buffer solution is added to a specimen and maintained at a prescribed temperature for a prescribed time. The pH of the mixture is lowered with an acid, the specimen is irradiated with an excitation light and the number of the living microbial cells is counted by detecting the light emitting from the cell.