FROZEN FERTILIZED EGG ENHANCED IN CONCEPTION RATE AND PREPARATION THEREOF
PURPOSE:To obtain the title fertilized egg capable of readily carrying out melting operation and surely transplanting without impairing existence of fertilized egg by bringing a solidified article of freeze-protecting substance in which the fertilized egg is packed into contact with a solidified art...
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creator | TAKEDA TETSUO |
description | PURPOSE:To obtain the title fertilized egg capable of readily carrying out melting operation and surely transplanting without impairing existence of fertilized egg by bringing a solidified article of freeze-protecting substance in which the fertilized egg is packed into contact with a solidified article of freeze- protecting substance-removing substance and superposing solidified articles. CONSTITUTION:A freeze protecting substance-removing agent (e.g. sugar solution) 7, air 6, freeze protecting substance solution (e.g. glycerin) 5 in which a fertilized egg is packed, air 4, freeze-protecting substance solution 3, air 2 and phosphoric acid buffer 1 are inserted from a cotton plug of straw tube into the straw tube in the above-mentioned order and the side opposite to the cotton plug is sealed by heating. Then the straw tube is put in a program freezer while turning the cotton plug part downward till a liquid layer of a solution 5 in which the fertilized egg is put is immersed and, preferably cooled to -10 deg.C and then shaken to move air 6 to the upper part of straw and then preferably cooled to |
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CONSTITUTION:A freeze protecting substance-removing agent (e.g. sugar solution) 7, air 6, freeze protecting substance solution (e.g. glycerin) 5 in which a fertilized egg is packed, air 4, freeze-protecting substance solution 3, air 2 and phosphoric acid buffer 1 are inserted from a cotton plug of straw tube into the straw tube in the above-mentioned order and the side opposite to the cotton plug is sealed by heating. Then the straw tube is put in a program freezer while turning the cotton plug part downward till a liquid layer of a solution 5 in which the fertilized egg is put is immersed and, preferably cooled to -10 deg.C and then shaken to move air 6 to the upper part of straw and then preferably cooled to <=-25 deg.C and further charged into a liquid nitrogen to provide the aimed fertilized egg.</description><language>eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; CARE OF BIRDS, FISHES, INSECTS ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; NEW BREEDS OF ANIMALS ; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR ; TRAPPING ; VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS</subject><creationdate>1991</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19911016&DB=EPODOC&CC=JP&NR=H03232441A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19911016&DB=EPODOC&CC=JP&NR=H03232441A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TAKEDA TETSUO</creatorcontrib><title>FROZEN FERTILIZED EGG ENHANCED IN CONCEPTION RATE AND PREPARATION THEREOF</title><description>PURPOSE:To obtain the title fertilized egg capable of readily carrying out melting operation and surely transplanting without impairing existence of fertilized egg by bringing a solidified article of freeze-protecting substance in which the fertilized egg is packed into contact with a solidified article of freeze- protecting substance-removing substance and superposing solidified articles. CONSTITUTION:A freeze protecting substance-removing agent (e.g. sugar solution) 7, air 6, freeze protecting substance solution (e.g. glycerin) 5 in which a fertilized egg is packed, air 4, freeze-protecting substance solution 3, air 2 and phosphoric acid buffer 1 are inserted from a cotton plug of straw tube into the straw tube in the above-mentioned order and the side opposite to the cotton plug is sealed by heating. Then the straw tube is put in a program freezer while turning the cotton plug part downward till a liquid layer of a solution 5 in which the fertilized egg is put is immersed and, preferably cooled to -10 deg.C and then shaken to move air 6 to the upper part of straw and then preferably cooled to <=-25 deg.C and further charged into a liquid nitrogen to provide the aimed fertilized egg.</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>CARE OF BIRDS, FISHES, INSECTS</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>NEW BREEDS OF ANIMALS</subject><subject>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</subject><subject>TRAPPING</subject><subject>VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1991</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPB0C_KPcvVTcHMNCvH08YxydVFwdXdXcPXzcPRzBnI8_RSc_YGsgBBPfz-FIMcQVwVHPxeFgCDXAEcgDyQY4uEa5OrvxsPAmpaYU5zKC6W5GRTdXEOcPXRTC_LjU4sLEpNT81JL4r0CPAyMjYyNTEwMHY2JUQMAJYktIQ</recordid><startdate>19911016</startdate><enddate>19911016</enddate><creator>TAKEDA TETSUO</creator><scope>EVB</scope></search><sort><creationdate>19911016</creationdate><title>FROZEN FERTILIZED EGG ENHANCED IN CONCEPTION RATE AND PREPARATION THEREOF</title><author>TAKEDA TETSUO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JPH03232441A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>1991</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>CARE OF BIRDS, FISHES, INSECTS</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>NEW BREEDS OF ANIMALS</topic><topic>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</topic><topic>TRAPPING</topic><topic>VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS</topic><toplevel>online_resources</toplevel><creatorcontrib>TAKEDA TETSUO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TAKEDA TETSUO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>FROZEN FERTILIZED EGG ENHANCED IN CONCEPTION RATE AND PREPARATION THEREOF</title><date>1991-10-16</date><risdate>1991</risdate><abstract>PURPOSE:To obtain the title fertilized egg capable of readily carrying out melting operation and surely transplanting without impairing existence of fertilized egg by bringing a solidified article of freeze-protecting substance in which the fertilized egg is packed into contact with a solidified article of freeze- protecting substance-removing substance and superposing solidified articles. CONSTITUTION:A freeze protecting substance-removing agent (e.g. sugar solution) 7, air 6, freeze protecting substance solution (e.g. glycerin) 5 in which a fertilized egg is packed, air 4, freeze-protecting substance solution 3, air 2 and phosphoric acid buffer 1 are inserted from a cotton plug of straw tube into the straw tube in the above-mentioned order and the side opposite to the cotton plug is sealed by heating. Then the straw tube is put in a program freezer while turning the cotton plug part downward till a liquid layer of a solution 5 in which the fertilized egg is put is immersed and, preferably cooled to -10 deg.C and then shaken to move air 6 to the upper part of straw and then preferably cooled to <=-25 deg.C and further charged into a liquid nitrogen to provide the aimed fertilized egg.</abstract><oa>free_for_read</oa></addata></record> |
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subjects | AGRICULTURE ANIMAL HUSBANDRY CARE OF BIRDS, FISHES, INSECTS FISHING FORESTRY HUMAN NECESSITIES HUNTING HYGIENE MEDICAL OR VETERINARY SCIENCE NEW BREEDS OF ANIMALS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR TRAPPING VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS |
title | FROZEN FERTILIZED EGG ENHANCED IN CONCEPTION RATE AND PREPARATION THEREOF |
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