FROZEN FERTILIZED EGG ENHANCED IN CONCEPTION RATE AND PREPARATION THEREOF

PURPOSE:To obtain the title fertilized egg capable of readily carrying out melting operation and surely transplanting without impairing existence of fertilized egg by bringing a solidified article of freeze-protecting substance in which the fertilized egg is packed into contact with a solidified art...

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description PURPOSE:To obtain the title fertilized egg capable of readily carrying out melting operation and surely transplanting without impairing existence of fertilized egg by bringing a solidified article of freeze-protecting substance in which the fertilized egg is packed into contact with a solidified article of freeze- protecting substance-removing substance and superposing solidified articles. CONSTITUTION:A freeze protecting substance-removing agent (e.g. sugar solution) 7, air 6, freeze protecting substance solution (e.g. glycerin) 5 in which a fertilized egg is packed, air 4, freeze-protecting substance solution 3, air 2 and phosphoric acid buffer 1 are inserted from a cotton plug of straw tube into the straw tube in the above-mentioned order and the side opposite to the cotton plug is sealed by heating. Then the straw tube is put in a program freezer while turning the cotton plug part downward till a liquid layer of a solution 5 in which the fertilized egg is put is immersed and, preferably cooled to -10 deg.C and then shaken to move air 6 to the upper part of straw and then preferably cooled to
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CONSTITUTION:A freeze protecting substance-removing agent (e.g. sugar solution) 7, air 6, freeze protecting substance solution (e.g. glycerin) 5 in which a fertilized egg is packed, air 4, freeze-protecting substance solution 3, air 2 and phosphoric acid buffer 1 are inserted from a cotton plug of straw tube into the straw tube in the above-mentioned order and the side opposite to the cotton plug is sealed by heating. Then the straw tube is put in a program freezer while turning the cotton plug part downward till a liquid layer of a solution 5 in which the fertilized egg is put is immersed and, preferably cooled to -10 deg.C and then shaken to move air 6 to the upper part of straw and then preferably cooled to &lt;=-25 deg.C and further charged into a liquid nitrogen to provide the aimed fertilized egg.</description><language>eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; CARE OF BIRDS, FISHES, INSECTS ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; NEW BREEDS OF ANIMALS ; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR ; TRAPPING ; VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS</subject><creationdate>1991</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=19911016&amp;DB=EPODOC&amp;CC=JP&amp;NR=H03232441A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=19911016&amp;DB=EPODOC&amp;CC=JP&amp;NR=H03232441A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TAKEDA TETSUO</creatorcontrib><title>FROZEN FERTILIZED EGG ENHANCED IN CONCEPTION RATE AND PREPARATION THEREOF</title><description>PURPOSE:To obtain the title fertilized egg capable of readily carrying out melting operation and surely transplanting without impairing existence of fertilized egg by bringing a solidified article of freeze-protecting substance in which the fertilized egg is packed into contact with a solidified article of freeze- protecting substance-removing substance and superposing solidified articles. 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CONSTITUTION:A freeze protecting substance-removing agent (e.g. sugar solution) 7, air 6, freeze protecting substance solution (e.g. glycerin) 5 in which a fertilized egg is packed, air 4, freeze-protecting substance solution 3, air 2 and phosphoric acid buffer 1 are inserted from a cotton plug of straw tube into the straw tube in the above-mentioned order and the side opposite to the cotton plug is sealed by heating. Then the straw tube is put in a program freezer while turning the cotton plug part downward till a liquid layer of a solution 5 in which the fertilized egg is put is immersed and, preferably cooled to -10 deg.C and then shaken to move air 6 to the upper part of straw and then preferably cooled to &lt;=-25 deg.C and further charged into a liquid nitrogen to provide the aimed fertilized egg.</abstract><oa>free_for_read</oa></addata></record>
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subjects AGRICULTURE
ANIMAL HUSBANDRY
CARE OF BIRDS, FISHES, INSECTS
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
HYGIENE
MEDICAL OR VETERINARY SCIENCE
NEW BREEDS OF ANIMALS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
TRAPPING
VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
title FROZEN FERTILIZED EGG ENHANCED IN CONCEPTION RATE AND PREPARATION THEREOF
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