SYNTHETIC PEPTIDE AND MEASURING KID AND MEASURING METHOD OF HUMAN MN-SUPEROXIDE DISMUTASE USING THE SAME PEPTIDE

NEW MATERIAL:A synthetic peptide of a monomer consisting of amino sequence expressed by the formula (X is 0-10 amino acid residues). USE:A reagent for measuring human Mn-superoxide dismutase (human Mn-SOD). PREPARATION:For example, alpha-amino protecting group is removed from N-tert.- butoxycarbonyl...

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Bibliographische Detailangaben
Hauptverfasser:	KAWAGUCHI TETSUO, NAKAJIMA YUKIO, TAKEYASU AKIRA, UDA TAIZO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	NEW MATERIAL:A synthetic peptide of a monomer consisting of amino sequence expressed by the formula (X is 0-10 amino acid residues). USE:A reagent for measuring human Mn-superoxide dismutase (human Mn-SOD). PREPARATION:For example, alpha-amino protecting group is removed from N-tert.- butoxycarbonyl-N'-2-chlorobenzyloxycarbonyl lysine resin used as a starting material by a solid phase method and then an amino acid is successively reacted with an amino acid from C end side according to amino acid sequence of peptide to synthesize a resin having peptide bond and the resultant resin is subjected to cleavage of protecting group and resin to provide the aimed synthetic peptide. Furthermore, a human Mn-SOD can be measured by carrying the synthetic peptide on a support and then competitively reacting the synthetic peptide with the human Mn-SOD and an enzyme labeled monoclonal antibody having high specificity in a measuring sample.