POLYPEPTIDE HAVING CELL ADHESION ACTIVITY

PURPOSE:To attain a cell adhesion activity comparable to that of human fibronectin(FN) by compounding a specified amino acid sequence. CONSTITUTION:A peptide composed of 283 amino acid residues and originated from FN is modified and prepared by genetic engineering technique and the Ser of the RGDS s...

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Hauptverfasser:	KAWASE YASUAKI, KIMIZUKA FUSAO, GOTO SHOICHI, ODATE YOICHI, KATOU IKUNOSHIN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To attain a cell adhesion activity comparable to that of human fibronectin(FN) by compounding a specified amino acid sequence. CONSTITUTION:A peptide composed of 283 amino acid residues and originated from FN is modified and prepared by genetic engineering technique and the Ser of the RGDS sequence which is the minimum active unit for the cell adhesion of the piptide composed of 279 amino acid residues (Pro -Met ) is converted to Val to obtain a polypeptide (A) having the amino acid sequence of formula and having cell adhesion activity. A plasmid pTFD707 coding the component A is introduced into E.coli BW313 to obtain a recombinant plasmid pTFD707-V (B). The component B is introduced into E.coli JM109 and the transformant E.coli JM109/pTF7321 (C) is cultured in a medium to obtain a cultured product (D). The microbial cells are collected from the component D and obtained cells are extracted and purified by ultrasonic treatment, dialysis, etc., to obtain the objective polypeptide having cell adhesion activity.