PRODUCTION OF GLYCOSIDE USING ENZYME

PURPOSE:To obtain a glycoside having surface activity and physiological activity by reacting a specific sugar donor and a sugar acceptor composed of a straight- chain aliphatic alcohol, etc., with beta-galactosidase in an aqueous system and/or an aqueous solution of an organic solvent. CONSTITUTION:...

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Hauptverfasser:	YOKOMICHI HIDESUE, WATARI TORU, NAKAMURA KAZUHIRO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To obtain a glycoside having surface activity and physiological activity by reacting a specific sugar donor and a sugar acceptor composed of a straight- chain aliphatic alcohol, etc., with beta-galactosidase in an aqueous system and/or an aqueous solution of an organic solvent. CONSTITUTION:A sugar donor (A) is selected from disaccharides or polysaccharides having galactose at the non-reducing terminal (e.g., galactose, lactose, etc.). A sugar acceptor (B) is selected from straight-chain, branched-chain or aliphatic alcohols such as methanol. A beta-galactosidase (C) originated from Aspergillus oryzae is selected to obtain a component C having high sugar transition activity and resistant to inactivation in organic solvent. The components A and B are dissolved at a molar ratio of 1:(5-50) in an aqueous system having a water-content of 10-70wt.% or in an aqueous solution of an organic solvent such as acetone to obtain a solution D. The component C is added to the component D and made to react at about pH5 and 5-70 deg.C for 3-500min to produce the objective glucoside.