JPH0249713B

PURPOSE:To impart a bacterial cell with the capability to produce heat-resistant leucine-dehydrogenase, by introducing a plasmid containing the gene of leucine-dehydrogenase derived from the chromosome DNA of a thermophilic microorganism into a bacterial cell which can be grown at normal temperature...

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Bibliographische Detailangaben
Hauptverfasser:	EZAKI NOBUYOSHI, OOSHIMA TOSHIHISA, SAKAMOTO YONEKAZU, NAGATA SHINJI, TANAKA HIDEHIKO, SODA KENJI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To impart a bacterial cell with the capability to produce heat-resistant leucine-dehydrogenase, by introducing a plasmid containing the gene of leucine-dehydrogenase derived from the chromosome DNA of a thermophilic microorganism into a bacterial cell which can be grown at normal temperature. CONSTITUTION:The gene of leucine-dehydrogenase derived from chromosome DNA is separated from the cell of bacterial strain belonging to thermophilic Bacillus genus, e.g. Bacillus stearothermophilus. The gene of the leucine-dehydrogenase and a DNA acting as a vector are digested with a restriction enzyme, and treated with a ligase to obtain plasmid pICRI having the gene of heat-resistant leucine-dehydrogenase. The capability to produce heat-resistant leucine-dehydrogenase can be imparted to a cell by introducing the plasmid to the cell of e.g. Escherichia coli.