PROTEINIC ESSENTIAL SUBSTANCE AND PRODUCTION THEREOF

NEW MATERIAL:A proteinic essential substance Ps-1 relating to manifestation of insulin secretion-enhancing activity having molecular weight: 28000+ or -1200, protein content: >=98wt.%, ratios of amino acid components (muM/100muM) of, Asp: 9.3+ or -1.0, Thr: 7.4+ or -0.8, Ser: 10.6+ or -1.0, Glu:...

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Bibliographische Detailangaben
Hauptverfasser:	MURAI TOSHI, ITO KIYOSHI, TAMURA MAKOTO, TOMIOKA CHIKANORI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	NEW MATERIAL:A proteinic essential substance Ps-1 relating to manifestation of insulin secretion-enhancing activity having molecular weight: 28000+ or -1200, protein content: >=98wt.%, ratios of amino acid components (muM/100muM) of, Asp: 9.3+ or -1.0, Thr: 7.4+ or -0.8, Ser: 10.6+ or -1.0, Glu: 10.6+ or -1.0, Pro: 4.4+ or -0.5, Gly: 11.2+ or -1.0, Ala: 10.6+ or -1.0, Cys/2: 1.3+ or -0.3, Val: 6.7+ or -0.7, Met: 1.6+ or -0.3, Ile: 3.2+ or -0.4, Leu: 5.5+ or -0.6, Tyr: 4.6+ or -0.5, Phe: 3.5+ or -0.4, Lys: 2.2+ or -0.3, His: 1.7+ or -0.3 and Arg: 5.9+ or -0.6, and isoelectric point: pH5.8. USE:Curing agent of diabetes. PREPARATION:For instance, Bordetella pertussis is cultured in shaking in medium and cultured solution is centrifuged to collect supernatant solution, then the solution is treated in hydroxyapatite column, thus adsorbed substance is eluted and the eluate is subjected to gel filtration, then purified to afford the aimed proteinic essential substance Ps-1.