METHOD FOR MEASURING FRUCTOSAMINE

PURPOSE:To make it possible to measure fructosamine by utilizing the oxidizing power of the oxidizing metal ions and/or their complexes such as CU, Fe, Co, Mn, and Ce on ascorbic acid. CONSTITUTION:In a method for determining fructosamine using tetrazolium salt, oxidizing metals and/or their complexes are added. As the complexes of the metal ions, the complexes of nitro-triacetic acid, ethylenediaminebiacetic acid and the like can be listed. In first reagent (100-mM carbonic acid buffer, 10.5 pH and 0.1-nM copper sulfate) of 1ml, 50-mul specimen is added. Reaction is performed for five minutes at 37 deg.C. Thereafter, second reagent (100-mM carbonic acid buffer, 10.5 pH and 1.25-mM nitroblue-tetrazolium) of 0.25ml is added, and reaction is performed at 37 deg.C. Absorbance change per minute (DELTAE/min) at 540nm is measured. The result is compared with a specimen wherein the ascorbin acid is added by 20mg/dl with reference to the specimen in which the ascorbin acid is not added. As a result, it is confirmed that the interference with the ascorbic acid is suppressed by the addition of the copper sulfate and the measurement can be performed in a short time.