ASSAY OF ALPHA-AMYLASE

PURPOSE:To accomplish assay in a short time with a small amount of enzyme of the title substance for use in acute pancreatitis diagnosis by determining saccharose with fructose-modified oligosaccharide as substrate, produced through glucoamylase or its combination with alpha-glucosidase. CONSTITUTIO...

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Bibliographische Detailangaben
Hauptverfasser:	OGAWA YOSHISUKE, NISHIMURA JUN, ICHINO MOTONOBU, KANEJIMA TOSHIKIMI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To accomplish assay in a short time with a small amount of enzyme of the title substance for use in acute pancreatitis diagnosis by determining saccharose with fructose-modified oligosaccharide as substrate, produced through glucoamylase or its combination with alpha-glucosidase. CONSTITUTION:A fructose-modified oligosaccharide (e.g. isopropylidene matotoheptaosyl frucside) as substrate is made to act on an alpha-amylase-contg, sample, and an enzyme reagent i.e., glucoamylase or its combination with alpha- glucosidase is then allowed to act on the resultant oligosaccharide to form saccharose. Thence, sucrose phosphorylase is made to act on this saccharose to form glucose-1-phosphoric acid and fructose, and this fructose is then allowed to react with NADH in the presence of mannitol dehydrogenase to produce NAD, which is then determined, thus accomplishing the objective assay of alpha-amylase activity.