JPH0214033B
PURPOSE:To prepare the titled substance (2-halo acid halidohydrolase) active to L-isomer and D-isomer of 2-halogenocarboxylic acid, from the cultured product obtained by the cultivation of bacteria belonging to Pseudomonas genus. CONSTITUTION:Bacteria belonging to Pseudomonas genus, e.g. Pseudomonas...
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| creator | EZAKI NOBUYOSHI MOTOSUGI KENZO SODA KENJI |
| description | PURPOSE:To prepare the titled substance (2-halo acid halidohydrolase) active to L-isomer and D-isomer of 2-halogenocarboxylic acid, from the cultured product obtained by the cultivation of bacteria belonging to Pseudomonas genus. CONSTITUTION:Bacteria belonging to Pseudomonas genus, e.g. Pseudomonas UK113 strain (FERM-P No.5666) are cultured aerobically in a conventional nutrient medium. The bacterial cells are disintegrated, and the fragments of the cell are removed therefrom to obtain a cell extraction liquid. The liquid is subjected to the salting-out, treatment with organic solvent, and chromatographic treatment to obtain isolated and purified 2-halo acid halidohydrolase. The 2-halogenocarboxylic acid halidohydrolase thus obtained converts the D-isomer and L-isomer of a |
| format | Patent |
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CONSTITUTION:Bacteria belonging to Pseudomonas genus, e.g. Pseudomonas UK113 strain (FERM-P No.5666) are cultured aerobically in a conventional nutrient medium. The bacterial cells are disintegrated, and the fragments of the cell are removed therefrom to obtain a cell extraction liquid. The liquid is subjected to the salting-out, treatment with organic solvent, and chromatographic treatment to obtain isolated and purified 2-halo acid halidohydrolase. The 2-halogenocarboxylic acid halidohydrolase thus obtained converts the D-isomer and L-isomer of a <=5C 2-halogenocarboxylic acid to the corresponding D-isomer and L- isomer of 2-hydroxy acid.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1990</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19900405&DB=EPODOC&CC=JP&NR=H0214033B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19900405&DB=EPODOC&CC=JP&NR=H0214033B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>EZAKI NOBUYOSHI</creatorcontrib><creatorcontrib>MOTOSUGI KENZO</creatorcontrib><creatorcontrib>SODA KENJI</creatorcontrib><title>JPH0214033B</title><description>PURPOSE:To prepare the titled substance (2-halo acid halidohydrolase) active to L-isomer and D-isomer of 2-halogenocarboxylic acid, from the cultured product obtained by the cultivation of bacteria belonging to Pseudomonas genus. CONSTITUTION:Bacteria belonging to Pseudomonas genus, e.g. Pseudomonas UK113 strain (FERM-P No.5666) are cultured aerobically in a conventional nutrient medium. The bacterial cells are disintegrated, and the fragments of the cell are removed therefrom to obtain a cell extraction liquid. The liquid is subjected to the salting-out, treatment with organic solvent, and chromatographic treatment to obtain isolated and purified 2-halo acid halidohydrolase. The 2-halogenocarboxylic acid halidohydrolase thus obtained converts the D-isomer and L-isomer of a <=5C 2-halogenocarboxylic acid to the corresponding D-isomer and L- isomer of 2-hydroxy acid.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1990</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZOD2CvAwMDI0MTA2duJhYE1LzClO5YXS3AxKbq4hzh66qQX58anFBYnJqXmpJfFIOpyMjIlSBABzqhwb</recordid><startdate>19900405</startdate><enddate>19900405</enddate><creator>EZAKI NOBUYOSHI</creator><creator>MOTOSUGI KENZO</creator><creator>SODA KENJI</creator><scope>EVB</scope></search><sort><creationdate>19900405</creationdate><title>JPH0214033B</title><author>EZAKI NOBUYOSHI ; MOTOSUGI KENZO ; SODA KENJI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JPH0214033BB23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>1990</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>EZAKI NOBUYOSHI</creatorcontrib><creatorcontrib>MOTOSUGI KENZO</creatorcontrib><creatorcontrib>SODA KENJI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>EZAKI NOBUYOSHI</au><au>MOTOSUGI KENZO</au><au>SODA KENJI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>JPH0214033B</title><date>1990-04-05</date><risdate>1990</risdate><abstract>PURPOSE:To prepare the titled substance (2-halo acid halidohydrolase) active to L-isomer and D-isomer of 2-halogenocarboxylic acid, from the cultured product obtained by the cultivation of bacteria belonging to Pseudomonas genus. CONSTITUTION:Bacteria belonging to Pseudomonas genus, e.g. Pseudomonas UK113 strain (FERM-P No.5666) are cultured aerobically in a conventional nutrient medium. The bacterial cells are disintegrated, and the fragments of the cell are removed therefrom to obtain a cell extraction liquid. The liquid is subjected to the salting-out, treatment with organic solvent, and chromatographic treatment to obtain isolated and purified 2-halo acid halidohydrolase. The 2-halogenocarboxylic acid halidohydrolase thus obtained converts the D-isomer and L-isomer of a <=5C 2-halogenocarboxylic acid to the corresponding D-isomer and L- isomer of 2-hydroxy acid.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | JPH0214033B |
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