JPH0214033B

PURPOSE:To prepare the titled substance (2-halo acid halidohydrolase) active to L-isomer and D-isomer of 2-halogenocarboxylic acid, from the cultured product obtained by the cultivation of bacteria belonging to Pseudomonas genus. CONSTITUTION:Bacteria belonging to Pseudomonas genus, e.g. Pseudomonas...

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Hauptverfasser:	EZAKI NOBUYOSHI, MOTOSUGI KENZO, SODA KENJI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To prepare the titled substance (2-halo acid halidohydrolase) active to L-isomer and D-isomer of 2-halogenocarboxylic acid, from the cultured product obtained by the cultivation of bacteria belonging to Pseudomonas genus. CONSTITUTION:Bacteria belonging to Pseudomonas genus, e.g. Pseudomonas UK113 strain (FERM-P No.5666) are cultured aerobically in a conventional nutrient medium. The bacterial cells are disintegrated, and the fragments of the cell are removed therefrom to obtain a cell extraction liquid. The liquid is subjected to the salting-out, treatment with organic solvent, and chromatographic treatment to obtain isolated and purified 2-halo acid halidohydrolase. The 2-halogenocarboxylic acid halidohydrolase thus obtained converts the D-isomer and L-isomer of a