JPH0137112B

JPH0137112B

PURPOSE:To obtain microorganism capable of producing 2-halo acid halidohydrolase and acting to the D-isomer of 2-halo acid. CONSTITUTION:Pseudomonas UK113 strain (FERM-P No.5666) belonging to Pseudomonas genus and capable of assimilating D-isomer of a 2-halo acid, is cultured in a medium containing...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: EZAKI NOBUYOSHI, MOTOSUGI KENZO, SODA KENJI
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE:To obtain microorganism capable of producing 2-halo acid halidohydrolase and acting to the D-isomer of 2-halo acid. CONSTITUTION:Pseudomonas UK113 strain (FERM-P No.5666) belonging to Pseudomonas genus and capable of assimilating D-isomer of a 2-halo acid, is cultured in a medium containing glycol, glycerine, etc. as carbon source, ammonium sulfate, ammonium chloride, etc. as nitrogen source, and salt of potassium, sodium, phosphoric acid, etc. as inorganic salt, pref. at 26-30 deg.C for about 5-48hr under aerobic conditions. The cultivation product can be used as an enzyme source as it is; however, the separated live bacterial cells, treated bacterial cells, as well as crude enzyme and purified enzyme can also be used as the enzyme source.