METHOD FOR DETERMINATING INORGANIC PHOSPHORUS AND REAGENT COMPOSITION FOR DETERMINATION

PURPOSE:To accurately determine an inorganic phosphorus in good precision, by blending a specimen with pyrophosphoric acid-dependent type phosphofruc tokinase, etc., and NAD or NADP and measuring the resultant NADH or NADPH. CONSTITUTION:Pyrophosphoric acid-dependent type phosphofructokinase is blen...

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Hauptverfasser:	YABUMOTO SHINICHI, KATAYAMA YOSHIAKI, SUMIYA MASANAGA, YAMAGUCHI SOICHIRO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To accurately determine an inorganic phosphorus in good precision, by blending a specimen with pyrophosphoric acid-dependent type phosphofruc tokinase, etc., and NAD or NADP and measuring the resultant NADH or NADPH. CONSTITUTION:Pyrophosphoric acid-dependent type phosphofructokinase is blended with phosphoglucoisomerase, fructose-1,6-bisphosphate, glucose-6- phosphatedehydrogenase and NAD or NADP to prepare a reagent composition for determining inorganic phosphorus. The prepared reagent composition is blended with a specimen and the resultant NADH or NADPH is measured to determine an inorganic phosphorus in the specimen. Furthermore, the above- mentioned total chemicals are added and blended at a time or addition of fructose-1,6-bisphosphate and phosphofructokinase can be separately carried out.