PRODUCTION OF ACYLNEURAMINATECYTIDYLTRANSFERASE
PURPOSE:To produce acylneuraminatecytidyltransferase, by culturing a microorganism belonging to a specific Escherichia genus and accumulating acylneuraminatecytidyltransferase in the cultured product. CONSTITUTION:A recombinant plasmid integrating chromosome DNA fragment containing acylneuraminatecy...
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| creator | MATSUI KATSUAKI UMETSU MASATOSHI KOBAYASHI KOUJI HARADA MOKICHI SUGIMOTO SEIKICHI |
| description | PURPOSE:To produce acylneuraminatecytidyltransferase, by culturing a microorganism belonging to a specific Escherichia genus and accumulating acylneuraminatecytidyltransferase in the cultured product. CONSTITUTION:A recombinant plasmid integrating chromosome DNA fragment containing acylneuraminatecytidyltransferase derived from acylneuraminatecytidyltransferase-producing bacterium belonging to the genus Escherichia is synthesized. The resultant recombinant plasmid is introduced into a microorganism belonging to the genus Escherichia to form transformed microorganism and the transformed microorganism is cultured in nutrient culture medium. Then acylneuraminatecytidyltransferase is accumulated in the cultured product and then recovered and purified to provide the acylneuraminatecytidyltransferase. |
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CONSTITUTION:A recombinant plasmid integrating chromosome DNA fragment containing acylneuraminatecytidyltransferase derived from acylneuraminatecytidyltransferase-producing bacterium belonging to the genus Escherichia is synthesized. The resultant recombinant plasmid is introduced into a microorganism belonging to the genus Escherichia to form transformed microorganism and the transformed microorganism is cultured in nutrient culture medium. Then acylneuraminatecytidyltransferase is accumulated in the cultured product and then recovered and purified to provide the acylneuraminatecytidyltransferase.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PRODUCTION OF ACYLNEURAMINATECYTIDYLTRANSFERASE |
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