ASSAY OF THYROID HORMONE AND REAGENT SYSTEM USED THEREFOR

PURPOSE: To directly measure thyroid gland hormone in serum or plasma by treating a sample by furosemide, substituting triopfpyjutpmomr (T3 ) and thyroxine (T4 ) from binding protein, and measuring the amount of substituted T3 and T4 . CONSTITUTION: A sample is treated by furosemide, T3 and T4 are s...

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Hauptverfasser: UIRIAMU AARU GUROSUKOTSUPU, ROBAATO KOWAARU, TOOMASU ARAN RAASON, BIRII JIEI GURIIN, POORU BII WAANKU JIYUNIA
Format: Patent
Sprache:eng
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Zusammenfassung:PURPOSE: To directly measure thyroid gland hormone in serum or plasma by treating a sample by furosemide, substituting triopfpyjutpmomr (T3 ) and thyroxine (T4 ) from binding protein, and measuring the amount of substituted T3 and T4 . CONSTITUTION: A sample is treated by furosemide, T3 and T4 are substituted from a binding protein, and the amount of substituted T3 and T4 is measured. The amount of the substituted T3 and T4 can be measured by using either various kinds of thyroid gland hormone assay reagents and methods. Also, the amount of forosemide to be used can be calculated as a function of the size of a sample to substitute essentially all T3 and T4 . Also, either fluorescence, absorption, fluorescent polarization, chemical lunescence, or radioactive means can be used as a detection method.