JP2928992B

Amplification of short single or double stranded nucleic acid fragments is effected in the presence of a primer pair, a buffer (pH 7-9.5) and a combination of a thermophilic DNA polymerase (P1) with proofreading activity, and a thermophilic DNA polymerase (P2) without proofreading activity, after a...

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Bibliographische Detailangaben
Hauptverfasser:	FURAI BURUUNO, KYUUBURAA HIRUDEGUNTO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Amplification of short single or double stranded nucleic acid fragments is effected in the presence of a primer pair, a buffer (pH 7-9.5) and a combination of a thermophilic DNA polymerase (P1) with proofreading activity, and a thermophilic DNA polymerase (P2) without proofreading activity, after a denaturing step in the case of double-stranded DNA fragments, chain extension is effected at least 70 degrees C for 30-240 sec.. Also claimed is an enzyme mixt. comprising polymerases of type P1 and P2 in a P1:P2 ratio of 1:10, where the concn. of the enzymes is calculated to give a final concn. of 2.0-3.0 U/volume of reaction mix.