JP2864285B
PURPOSE:To improve safety of food by transforming host bacterium with a specific vector. CONSTITUTION:Replication onset range (ori) derived from plasmid pLJI of Lactobacillus helveticus is linked with replication onset range derived from plasmid pBR329 of Escherichia coli and beta-galactosidase gene...
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| creator | HASHIBA EN AOYAMA KENJI TAKIGUCHI RYUICHI |
| description | PURPOSE:To improve safety of food by transforming host bacterium with a specific vector. CONSTITUTION:Replication onset range (ori) derived from plasmid pLJI of Lactobacillus helveticus is linked with replication onset range derived from plasmid pBR329 of Escherichia coli and beta-galactosidase gene derived from Lactobacillus bulgaricus to constitute complex shuttle vector pBH9 (A) for lactic acid bacillus-Escherichia coli. Then a desired range derived from plasmid pAMbeta1 of Enterococcus faecalis is integrated into an upper stream of beta- galactosidase gene of the component A to give a vector (B) strongly manifesting beta-galactosidase gene as a selective marker. Then a lactose nonassimilating host lactic acid vacillus is transformed with the component B to collect Lactobacillus helveticus SBT2,196 strain (FERM P-11,676). |
| format | Patent |
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CONSTITUTION:Replication onset range (ori) derived from plasmid pLJI of Lactobacillus helveticus is linked with replication onset range derived from plasmid pBR329 of Escherichia coli and beta-galactosidase gene derived from Lactobacillus bulgaricus to constitute complex shuttle vector pBH9 (A) for lactic acid bacillus-Escherichia coli. Then a desired range derived from plasmid pAMbeta1 of Enterococcus faecalis is integrated into an upper stream of beta- galactosidase gene of the component A to give a vector (B) strongly manifesting beta-galactosidase gene as a selective marker. 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| language | eng |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | JP2864285B |
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