JP2864285B

PURPOSE:To improve safety of food by transforming host bacterium with a specific vector. CONSTITUTION:Replication onset range (ori) derived from plasmid pLJI of Lactobacillus helveticus is linked with replication onset range derived from plasmid pBR329 of Escherichia coli and beta-galactosidase gene...

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Bibliographische Detailangaben
Hauptverfasser:	HASHIBA EN, AOYAMA KENJI, TAKIGUCHI RYUICHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To improve safety of food by transforming host bacterium with a specific vector. CONSTITUTION:Replication onset range (ori) derived from plasmid pLJI of Lactobacillus helveticus is linked with replication onset range derived from plasmid pBR329 of Escherichia coli and beta-galactosidase gene derived from Lactobacillus bulgaricus to constitute complex shuttle vector pBH9 (A) for lactic acid bacillus-Escherichia coli. Then a desired range derived from plasmid pAMbeta1 of Enterococcus faecalis is integrated into an upper stream of beta- galactosidase gene of the component A to give a vector (B) strongly manifesting beta-galactosidase gene as a selective marker. Then a lactose nonassimilating host lactic acid vacillus is transformed with the component B to collect Lactobacillus helveticus SBT2,196 strain (FERM P-11,676).