JP2516737B

PURPOSE:To provide a plasmid vector having a specific promoter dimer, and capable of expressing the inserted DNA in a culture animal cell irrespective of the insertion direction of the exogeneous DNA. CONSTITUTION:A plasmid containing SV40 early promoter and replication initiation point is digested...

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Hauptverfasser:	HAMAOKA TOSHUKI, SUGANO HARUO, TANIGUCHI KOREAKI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To provide a plasmid vector having a specific promoter dimer, and capable of expressing the inserted DNA in a culture animal cell irrespective of the insertion direction of the exogeneous DNA. CONSTITUTION:A plasmid containing SV40 early promoter and replication initiation point is digested with a restriction enzyme to obtain a DNA fragment having blunt end at the downstream end of the promoter. The fragment is introduced into Escherichia coli to obtain a transformed strain containing said DNA fragment. pKCR(Hpa) is separated from the E.coli, and a promoter DNA fragment is separated therefrom. Two promoter DNA fragments are bonded with an adaptor DNA. The plasmid pML-RIIG is modified to produce a vector for the insertion of the promoter dimer, and the early promoter is introduced into the vector to obtain a plasmid pDE-1.