CELL CULTURE SUBSTRATE, AND METHOD FOR PRODUCING THE SAME

To provide a cell culture substrate that has a uniform particle diameter and is capable of peeling and recovering a cell aggregate without a temperature change after culture, and a method for producing the cell culture substrate.SOLUTION: A cell culture substrate has a substrate having irregularity on a surface and a hydrophilic polymer layer covering the substrate along the irregularity, where the maximum height in a reference length 10 μm of the surface irregularity that the cell culture substrate has is 0.1 to 2 μm, the cell culture substrate has the following two regions (A) and (B), and the regions (A) are plasma treatment regions. (A) island-shaped regions having an area of 0.001 to 5 mm2 which has cell adhesiveness and cell proliferating property. (B) a region which is adjacent to the regions (A) and has no cell proliferating property.SELECTED DRAWING: Figure 4 【課題】粒径が均一で、培養後に温度変化を伴うことなく細胞凝集塊を剥離回収可能な細胞培養基材及び該細胞培養基材の製造方法を提供する。【解決手段】表面に凹凸を有する基材と、上記基材を上記凹凸に沿って被覆する親水性高分子層とを有する細胞培養基材であって、上記細胞培養基材が有する表面凹凸の基準長さ１０μｍにおける最大高さが０．１～２μｍであり、上記細胞培養基材は下記（Ａ）及び（Ｂ）の２つの領域を有し、上記（Ａ）領域はプラズマ処理領域である、細胞培養基材。（Ａ）細胞接着性及び細胞増殖性を有する面積０．００１～５ｍｍ２の島状の領域（Ｂ）上記（Ａ）領域に隣接し、細胞増殖性を有しない領域【選択図】図４