GANODERMA LUCIDUM β-GLUCAN EXTRACT, AND PREPARATION METHOD AND DETECTION METHOD THEREFOR

To provide a Ganoderma lucidum β-glucan extract, and a preparation method and detection method therefor.SOLUTION: The preparation method includes: a step S1 of crushing fruiting bodies of Ganoderma lucidum, mixing the crushed fruiting bodies with a solvent, adding trypsin to perform enzymatic digestion for 0.5-2 h, and separating to obtain a Ganoderma lucidum fruiting body residue; a step S2 of mixing the Ganoderma lucidum fruiting body residue with an alkali solution, performing ultrasonic extraction under a heating condition, adjusting the pH value of the obtained filtrate to be neutral, and concentrating to obtain a crude Ganoderma lucidum polysaccharide; a step S3 of performing alcoholysis of the crude Ganoderma lucidum polysaccharide, separating the obtained precipitate, and freeze-drying to obtain a crude Ganoderma lucidum β-glucan extract; and a step S4 of purifying the crude Ganoderma lucidum β-glucan extract by glucose gel column chromatography to obtain a Ganoderma lucidum β-glucan extract. The preparation method is simple, has high product purity and high extraction efficiency. The detection method is simple, rapid, sensitive, and accurate.SELECTED DRAWING: None 【課題】本発明は、霊芝β－グルカン抽出物およびその調製方法並びに検出方法を提供する。【解決手段】この調製方法は、Ｓ１．霊芝実物を粉砕して溶媒と混合した後、トリプシンを加えて０．５～２ｈ酵素消化し、分離して霊芝実物残渣を得る工程と、Ｓ２．霊芝実物残渣をアルカリ水溶液と混合し、加熱条件下で超音波抽出し、得られたろ液のｐＨ値を中性に調整して濃縮して霊芝粗製多糖を得る工程と、Ｓ３．霊芝粗製多糖をアルコール分解し、得られた沈殿物を分離、凍結乾燥して霊芝β－グルカン粗抽出物を得る工程と、Ｓ４．霊芝β－グルカン粗抽出物をグルコースゲルカラムクロマトグラフィーで精製して霊芝β－グルカン抽出物を得る工程と、を含む。本発明の調製方法は、簡単で、生成物の純度が高く、抽出効率が高く、検出方法が簡単で迅速であり、高感度で正確である。【選択図】なし