SAMPLE PRETREATMENT METHOD USING MAGNETIC PARTICLES

To improve the efficiency of an analysis of components in a liquid by efficiently and rapidly removing substances interfering with analysis that are present in the same liquid, e.g. erythrocytes that interfere when blood is subjected to various types of analysis when analyzing components in a liquid sample.SOLUTION: In a liquid containing a substance to be analyzed and an analytical disturbing substance, magnetic particles having a functional group that acts on the analysis disturbing substance are dispersed. After forming a complex between the magnetic particles and the analysis disturbing substance, the resulting complex is magnetized to a magnet and the resulting supernatant is separated. Thereby, an analysis liquid is obtained from which the interfering substances have been removed. For example, if erythrocytes in the blood are to be removed, if positively charged groups such as an amino group and an ammonium group are employed as a functional group to be employed, it efficiently causes the aggregation of erythrocytes, which are then magnetized together with the magnetic particles. In addition, many of the substances to be analyzed are not affected, so there is almost no adverse effect on the results of the analysis.SELECTED DRAWING: None 【課題】 液体試料中の成分の分析に際して、同じ液体中に存在している分析の妨害物質、例えば血液を各種分析に供する際に妨害となる赤血球を効率的かつ迅速に除去し、液中成分の分析の効率を向上させる。【解決手段】 分析対象物質および分析妨害物質が含まれる液体中に、当該分析妨害物質と作用する官能基を有する磁性粒子を分散させ、磁性粒子と分析妨害物質との複合体を形成させた後、生じた複合体を磁石に磁着させ、生じた上清を分離することで、妨害物質が除かれた分析用の液体が得られる。採用する官能基としては、例えば血液中の赤血球を除くのであれば、アミノ基、アンモニウム基等の正帯電性の基を採用すれば、効率的に赤血球の凝集を引き起こし、磁性粒子と共に磁着する。かつ分析対象物質の多くは影響を受けないため、分析結果に悪影響を与えることはほぼない。【選択図】 なし