METHOD FOR PRODUCING VANILLIN

To provide a mutated aromatic carboxylic acid reductase (ACAR) useful for production of vanillin; and a method for producing vanillin using the same.SOLUTION: Vanillin is produced by using a mutated ACAR having mutation in an amino acid residue such as C28, C135, R241, G249, R252, W259, M265, V270,...

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Bibliographische Detailangaben
Hauptverfasser:	TAKAKURA JUN, ODA YUKI, TAKAHASHI KAZUTOSHI, TASHIRO AKARI, NAKADA KUNIO, FUKUI KEITA, KASHIWAGI TATSUMI, TAGAMI UNO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a mutated aromatic carboxylic acid reductase (ACAR) useful for production of vanillin; and a method for producing vanillin using the same.SOLUTION: Vanillin is produced by using a mutated ACAR having mutation in an amino acid residue such as C28, C135, R241, G249, R252, W259, M265, V270, M271, S274, T275, Q278, Q310, V312, I353, S356, I376, E377, G378, Y379, S381, T382, A384, A385, G386, V387, S388, I389, Y467, I574 .SELECTED DRAWING: None 【課題】バニリンの生産に有用な変異型芳香族カルボン酸レダクターゼ（aromatic carboxylic acid reductase；ACAR）およびそれを用いたバニリンの製造方法を提供する。【解決手段】C28、C135、R241、G249、R252、W259、M265、V270、M271、S274、T275、Q278、Q310、V312、I353、S356、I376、E377、G378、Y379、S381、T382、A384、A385、G386、V387、S388、I389、Y467、I574等のアミノ酸残基に変異を有する変異型ACARを利用してバニリンを製造する。【選択図】なし