METHOD FOR PRODUCING REGENERATED HAIR FOLLICLE PRIMORDIUM

To provide a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.SOLUTION: Provided is a method for producing a regenerated hair follicle primordium comp...

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Hauptverfasser: TSUJI TAKASHI, TOYOSHIMA KIMIE, MORI HISASHI
Format: Patent
Sprache:eng ; jpn
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Zusammenfassung:To provide a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.SOLUTION: Provided is a method for producing a regenerated hair follicle primordium comprising culturing a first cell population containing epithelial cells and a second cell population containing mesenchymal cells while bringing them into contact with each other to obtain a hair follicle primordium. The production method of the present invention is characterized in that at least one of the first cell population and the second cell population has formed a cell aggregate before the contact and no culture support is used when bringing the other cell population into contact with the cell aggregate.SELECTED DRAWING: None 【課題】 従来の製造方法に比して、簡易かつ大量に再生毛包原基の製造を可能にする、再生毛包原基の製造方法を提供すること。【解決手段】 上皮系細胞を含む第1の細胞集団と、間葉系細胞を含む第2の細胞集団とを接触させながら培養することにより、毛包原基を得る工程を含む、再生毛包原基の製造方法が提供される。本発明の製造方法において、前記第1の細胞集団および前記第2の細胞集団の少なくともいずれか一方は、前記接触の前に細胞凝集体を形成しており、前記細胞凝集体に対して他方の細胞集団を接触させる際、培養支持体を使用しないことを特徴とする。【選択図】 なし