METHOD FOR MAKING CAR-T CELL, KIT FOR MAKING CAR-T CELL, AND METHOD FOR IMPROVING CAR-T CELL CONTENT

To provide a method for making CAR-T cells without using feeder cells, a cell making kit that is used in the method, and a method for improving a CAR-T cell content.SOLUTION: A method for making CAR-T cells according to one embodiment of the present invention includes causing chimera antibody recept...

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Hauptverfasser:	AKABOSHI HIDEKAZU, NOZAKI EMI, ISHIHARA MITSUKO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a method for making CAR-T cells without using feeder cells, a cell making kit that is used in the method, and a method for improving a CAR-T cell content.SOLUTION: A method for making CAR-T cells according to one embodiment of the present invention includes causing chimera antibody receptor (CAR) gene-transduced cells to secrete CAR antigen proteins or their partial proteins extracellularly, thereby making a cell group with a high CAR-T cell content without using feeder cells.SELECTED DRAWING: Figure 1 【課題】フィーダー細胞を用いることなく、CAR-T細胞を作製する方法、その方法に使用される細胞作製用キット及びCAR-T細胞含有率向上方法を提供する。【解決手段】実施形態に従うCAR-T細胞を作製する方法は、キメラ抗体受容体（CAR）遺伝子導入細胞からCAR抗原タンパク質又はその部分タンパク質を細胞外に分泌させることにより、フィーダー細胞を用いずにCAR-T細胞含有率の高い細胞群を作ることを備える。【選択図】図１