METHOD FOR PRODUCING PROLIFERATED CELLS, METHOD FOR PRODUCING CELL PRODUCT, MESENCHYMAL STEM CELL POPULATION AND METHOD FOR PRODUCING THE SAME, CULTURE SUPERNATANT OF STEM CELLS AND METHOD FOR PRODUCING THE SAME, AND THERAPEUTIC AGENT

To provide a mesenchymal stem cell population that has the proportion of HLA-ABC positive mesenchymal stem cells reduced to 70% or less, by a cell culture technique that enables cells seeded at a low cell density to be proliferated at a high proliferation rate.SOLUTION: A method includes culturing mesenchymal stem cells, which have been seeded at a cell density of 0.002 to 1000 cells/cm2, through adhesion culture in a proliferation culture medium in the presence of a culture substrate selected from a laminin fragment having integrin-binding activity and a modified form thereof, thereby yielding a mesenchymal stem cell population that has the proportion of HLA-ABC positive cells reduced to 70% or less compared to the pre-cultured mesenchymal stem cell population.SELECTED DRAWING: Figure 13 【課題】低い細胞密度で播種された細胞を高い増殖倍率で増殖させることができる細胞培養技術に基づく、ＨＬＡ－ＡＢＣ陽性の間葉系幹細胞の比率が７０％以下である間葉系幹細胞集団を提供すること。【解決手段】０．００２～１０００細胞／ｃｍ２の細胞密度で播種された間葉系幹細胞を、増殖培養液中で、インテグリン結合活性を有するラミニンフラグメントおよびその改変体から選ばれる培養基質の存在下において、接着培養により培養して、培養前の間葉系幹細胞集団と比べて、ＨＬＡ－ＡＢＣ陽性の比率が７０％以下に低下した間葉系幹細胞集団を得る。【選択図】図１３