ENGINEERED CAS9 NUCLEASES

To provide Cas9 nuclease variants and methods of producing and using such variants.SOLUTION: The present invention provides an isolated Staphylococcuspyogenes Cas9 (SPCas9) polypeptide comprising an amino acid substitution, relative to a wild-type SPCas9, at one or more of the following positions: D...

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Bibliographische Detailangaben
Hauptverfasser:	DEREK CERCHIONE, BARRETT ETHAN STEINBERG
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide Cas9 nuclease variants and methods of producing and using such variants.SOLUTION: The present invention provides an isolated Staphylococcuspyogenes Cas9 (SPCas9) polypeptide comprising an amino acid substitution, relative to a wild-type SPCas9, at one or more of the following positions: D23, D1251, Y128, T67, N497, R661, Q695, and/or Q926.SELECTED DRAWING: None 【課題】本開示は、Ｃａｓ９ヌクレアーゼ変異体、並びにそのような変異体を生成する方法及び用いる方法を提供する。【解決手段】野生型化膿レンサ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓｐｙｏｇｅｎｅｓ）Ｃａｓ９（ＳＰＣａｓ９）と比較して、以下の位置：Ｄ２３、Ｄ１２５１、Ｙ１２８、Ｔ６７、Ｎ４９７、Ｒ６６１、Ｑ６９５、及び／又はＱ９２６の１つ以上にてアミノ酸置換を含む、単離ＳＰＣａｓ９ポリペプチドを提供する。【選択図】なし