MUTANT CIS-PRENYLTRANSFERASE (CPT) FAMILY PROTEIN, METHOD FOR PRODUCING POLYISOPRENOID, VECTOR, TRANSGENIC PLANT, METHOD FOR PRODUCING PNEUMATIC TIRE, AND METHOD FOR PRODUCING RUBBER PRODUCT

To provide a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid.SOLUTION: Provided herein is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating an amino acid sequen...

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Hauptverfasser:	YAMASHITA SATORU, YAMAGUCHI HARUHIKO, TOZAWA YUZURU, NAKAYAMA TORU, INOUE (MIYAGI) YUKINO, TAKAHASHI SEIJI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OF MACROMOLECULAR COMPOUNDS COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY GENERAL PROCESSES OF COMPOUNDING HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC MACROMOLECULAR COMPOUNDS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP TRAPPING VINEGAR WINE WORKING-UP
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Zusammenfassung:	To provide a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid.SOLUTION: Provided herein is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating an amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to an amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles and mutating an amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein not found on the rubber particles to be identical or similar to an amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on the rubber particles.SELECTED DRAWING: Figure 1 【課題】高分子量のポリイソプレノイドの製造を可能にする、変異シス型プレニルトランスフェラーゼ（ＣＰＴ）ファミリー蛋白質、ポリイソプレノイドの製造方法を提供する。【解決手段】ゴム粒子上に存在しないシス型プレニルトランスフェラーゼ（ＣＰＴ）ファミリー蛋白質のＮ末端領域のアミノ酸配列を、ゴム粒子上に存在するシス型プレニルトランスフェラーゼ（ＣＰＴ）ファミリー蛋白質のＮ末端領域のアミノ酸配列と同一に又は類似するように変異し、前記ゴム粒子上に存在しないシス型プレニルトランスフェラーゼ（ＣＰＴ）ファミリー蛋白質のＣ末端領域のアミノ酸配列を、ゴム粒子上に存在するシス型プレニルトランスフェラーゼ（ＣＰＴ）ファミリー蛋白質のＣ末端領域のアミノ酸配列と同一に又は類似するように変異した変異シス型プレニルトランスフェラーゼ（ＣＰＴ）ファミリー蛋白質。【選択図】図１