OLIGONUCLEOTIDE PROBE FOR TARGET NUCLEIC ACID DETECTION

To provide a probe with a stem-loop structure capable of detecting a target nucleic acid with high sensitivity.SOLUTION: The above problem is solved by setting the base sequence of a stem region to one of (i) CCCTgTg on one end and CACAggg on the other end, (ii) CCCTgTgTg on one end and CACACAggg on...

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Hauptverfasser:	HIDENO TOMOHIRO, KOBAYASHI SHUHO, MAKINO YURIKO, HATTORI ATSUNORI, JIDO YUMIKO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a probe with a stem-loop structure capable of detecting a target nucleic acid with high sensitivity.SOLUTION: The above problem is solved by setting the base sequence of a stem region to one of (i) CCCTgTg on one end and CACAggg on the other end, (ii) CCCTgTgTg on one end and CACACAggg on the other end, and (iii) CTCggg on one end and CCCgAg on the other end.SELECTED DRAWING: None 【課題】標的核酸を高感度に検出可能なステムループ構造を有したプローブを提供すること。【解決の手段】ステム領域の塩基配列を、（ｉ）一方の末端がＣＣＣＴｇＴｇであり他方の末端がＣＡＣＡｇｇｇ、（ｉｉ）一方の末端がＣＣＣＴｇＴｇＴｇであり他方の末端がＣＡＣＡＣＡｇｇｇ、および（ｉｉｉ）一方の末端がＣＴＣｇｇｇであり他方の末端がＣＣＣｇＡｇのいずれかにすることで、前記課題を解決する。【選択図】なし