EXOSOME PRODUCTION METHOD AND EXOSOME MANUFACTURING METHOD

To promote the production of exosomes from cells and efficiently manufacture the exosomes.SOLUTION: The present invention relates to exosome production methods and manufacturing methods to promote the production of exosomes from cells cultured in a medium. To the medium for culturing the cells, nico...

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Hauptverfasser:	NAKADA MITSUTAKA, MIWA AKIHIRO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To promote the production of exosomes from cells and efficiently manufacture the exosomes.SOLUTION: The present invention relates to exosome production methods and manufacturing methods to promote the production of exosomes from cells cultured in a medium. To the medium for culturing the cells, nicotinamide mononucleotide is added with its concentration ranging from 1 nmol/L to 80 nmol/L. After the cells are cultured in a culture vessel, the medium is recovered from the culture vessel, and exosomes are extracted from the medium.SELECTED DRAWING: Figure 4 【課題】細胞からのエクソソームの産生を促進させ、効率的にエクソソームを製造することを目的とする。【解決手段】培地中において培養している細胞からのエクソソームの産生を促進させるエクソソーム産生方法および製造方法に関する。上記細胞を培養する培地に、１ｎｍｏｌ／Ｌ～８０ｎｍｏｌ／Ｌの濃度範囲となるようにニコチンアミドモノヌクレオチドを添加する。培養容器内で細胞を培養した後、当該培養容器から培地を回収して、当該培地からエクソソームを抽出する。【選択図】図４