CELL PRESERVATION METHOD

To provide cell suspension with low cryoprotective agent concentration.SOLUTION: This method of preserving cells comprises the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, and (b) freezing the enriched fracti...

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Bibliographische Detailangaben
Hauptverfasser:	YANAGIDA YASUTOMO, ISHIDAO TAKEFUMI, MINAMI KAZUNARI, RINOIE CHUGO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide cell suspension with low cryoprotective agent concentration.SOLUTION: This method of preserving cells comprises the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, and (b) freezing the enriched fraction to prepare a frozen material. It is also possible to use a method of producing a cell suspension, comprising the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, (b) freezing the enriched fraction to prepare a frozen material, (c) thawing the frozen material to prepare a thawed material, and (d) mixing the thawed material and a solution to produce a cell suspension.SELECTED DRAWING: None 【課題】凍結保護剤の濃度が低い細胞懸濁液を提供する。【解決手段】(a)細胞と凍結保護液とを含有する細胞懸濁液から細胞を濃縮し、濃縮画分を生成する工程、及び(b)前記濃縮画分を凍結し、凍結物を生成する工程、を含む、細胞の保存方法を用いる。又は、(a)細胞と凍結保護液とを含有する細胞懸濁液から細胞を濃縮し、濃縮画分を生成する工程、(b)前記濃縮画分を凍結し、凍結物を生成する工程、(c)前記凍結物を解凍し、解凍物を生成する工程、及び(d)前記解凍物と溶液を混合し、細胞懸濁液を生成する工程、を含む、細胞懸濁液の生産方法を用いてもよい。【選択図】なし